PRODUCTION CHARACTERIZATION AND OPTIMIZATION OF NATTOKINASE

Selection of this research work was initiated from patients those have DVT (Deep Vein Thrombosis), Heart Disease, Arteriosclerosis etc.

PRODUCTION  CHARACTERIZATION AND OPTIMIZATION OF NATTOKINASE

PRODUCTION CHARACTERIZATION AND OPTIMIZATION OF NATTOKINASE

The present research work was related to few problems; those were related to near about all sectors. As per the quote “Need is the Mother of Invention”, we partially solved the problem of above mention sectors through our research. Selection of this research work was initiated from patients those have DVT (Deep Vein Thrombosis), Heart Disease, Arteriosclerosis etc. Nattokinase (subtilisin NAT, NK), a potent fibrinolytic enzyme, was primarily isolated from a traditional fermented food “natto” in Japan. The enzyme is a subtilisin-like serine protease composed of 275 amino acid residues and has a molecular weight of 27.7 kDa. Fermented cheese-like food, Natto has been formed by fermentation by inoculating the bacterium Bacillus natto in the boiled soybeans. The resulting nattokinase enzyme is produced when the bacterium acts on the soybeans (Sumi et al., 1987). Nattokinase is a serine end peptidase with a molecular weight of 20,000 Daltons and having isoelectric point (pI) is 8.6. Nattokinase potentiates fibrinolytic activity. In other words, it could dissolve fibrin. Fibrin is a protein involved in the clotting of blood that is polymerized to form a "mesh" that forms a haemostatic plug or clot (in conjunction with platelets). Fibrin is made from fibrinogen and it is a soluble plasma glycoprotein synthesized by the liver. Nattokinase prevents aggregation of red blood cells. Specifically, Nattokinase supports the fibrinolytic clearing system in the body which is responsible for healthy circulation, by helping to break down fibrin protein deposits in the bloodstream to promote and enhance healthy blood flow. Nattokinase facilitates blood flow contributing to healthy circulation and balance to maintain normal blood pressure.

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PRODUCTION, CHARACTERIZATION AND OPTIMIZATION OF NATTOKINASE
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The present research work was related to few problems; those were related to near about all sectors. As per the quote “Need is the Mother of Invention”, we partially solved the problem of above mention sectors through our research. Selection of this research work was initiated from patients those have
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Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, focusing on industrial biotechnology and bioengineering practices for the production of industrial products, such as enzymes, organic acids, biopolymers, and biosurfactants, and the processes for
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Showcases the recent advances in microbial functional food applications across food science, microbiology, biotechnology, and chemical engineering Microbial technology plays a key role in the improvement of biotechnology, cosmeceuticals, and biopharmaceutical applications. It has turned into a subject of expanding significance because new microbes and their related biomolecules are distinguished

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